. Author manuscript; available in PMC: 2008 Sep 12.

Published in final edited form as: Biochemistry. 2005 Aug 9;44(31):10457–10465. doi: 10.1021/bi050283d

Table 3.

Reduction rates of wild type and R481K CcO from Global analysis. Na-Dithionite was rapidly mixed with wild type or R481K CcO in a stopped-flow rapid scanning spectrophotometer (OLIS rsm-16) (see Figure 5). Global fitting of the resulting spectral scans from the averaging of at least 2 separate measurements of 3 data sets gave kinetic rates (k_obs s^-1) of reduction for each of the hemes. Standard deviations given are from the averaging of data sets.

	Rates of Reduction (k_obs s^-1)
	pH6.5		pH8
CcO	Heme a	Heme a₃	Heme a	Heme a₃
Wild-type	6.42 ±.78	0.49 ±.09	2.09 ±.54	0.22 ±.04
R481K	6.10 ±.30	0.11 ±.04	1.52 ±.20	0.05 ±.01
D132A	6.19 ± .79	0.48 ± .07	2.24 ± .16	0.23 ±.06
D132A/R481K	2.51 ± .18	0.18 ± 00	1.34 ± .02	0.09 ± .01