Figure 4.

Dependence of pK_d on ionic strength. Dissociation constants of G3P-yTIM were determined as a function of ionic strength of solution using NaCl in the presence of 55 mM TEA buffer, pH 7.6. The solid line is the fit to equation 5, with I as the ionic strength of solutions of NaCl (filled circles), and $Z_P^2,Z_L^2$, and $Z_{PL}^2$ represent the square of the charges on protein, ligand and complex, filled triangle (NaF), filled diamond (NaI), open circle (LiCl), open triangle (CaCl₂).