Sequence alignments A) PICS module 2 DH domain, conserved His in boldface: PICS_DH2, PikAI, aa 3588–3647, UniProtKB/TrEMBL entry Q9ZGI5_9ACTO; DEBS_DH4, EryAII, aa 2386–2445, Q5UNP5_SACER; RAPS_DH7, RapB, aa 4130–4390, Q54296_STRHY; RIFS_DH4, RifB, aa 932–992, O52545_AMYMD; PICS_DH4, PikAII, aa 2437–2497, Q9ZGI4_9ACTO; TYLS_DH2, TylG, aa 3523–3583, O33954_STRFR. B) PICS module 2 KR domain, conserved Lys, Ser, and Tyr in boldface: PICS_KR2, PikAI, aa 4202–4260, Q9ZGI5_9ACTO; TYLS_KR2, TylG, aa 4119–4175, O33954_STRFR; RIFS_KR3, RifA, aa 1927–1983, O54666_AMYMD; DEBS_KR4, EryAII, aa 3242–3297, EryAII, Q5UNP5_SACER; PICS_KR5, PikAIII, aa 1217–1273, Q9ZGI3_9ACTO; DEBS_KR1, EryAI, aa 1767–1823, Q5UNP6_SACER. Alignments generated using CLUSTALW at PBIL (http://pbil.univ-lyon1.fr/) (Ref. Combet, C.; Blanchet,C.; Geourjon, C.; Deléage, G. TIBS 2000, 25, 147–150.)