. Author manuscript; available in PMC: 2008 Sep 12.

Published in final edited form as: J Am Chem Soc. 2005 Dec 14;127(49):17393–17404. doi: 10.1021/ja055672+

Table 1.

Steady-state kinetic parameters for PICS module 2+TE and mutants.

enzyme/mutant	product	substrate	K_m (mM)	k_cat (min⁻¹)
PICS module 2+TE	5	4	1.6±0.3	0.23±0.02
	5	malonyl-CoA	0.44±0.06	0.23±0.02
	10	4	1.2±0.4	0.49±0.04
	10	malonyl-CoA	0.35±0.07	0.49±0.04
PICS module 2+TE (-NADPH)	7	4	7.7±4.5	0.15±0.04
PICS module 2+TE (-NADPH)	7	malonyl-CoA	0.40±0.17	0.15±0.04
DH⁰ - H3611F	10	4	17.0±3.5	0.44±0.06
DH⁰ - H3611F	10	malonyl-CoA	0.25±0.07	0.44±0.06
KR⁰ - S4237A	7	4	2.9±2.0	0.31±0.19
KR⁰ – Y4250F	7	4	3.9±1.3	0.32±0.06
KR⁰ – K4211Q	7	4	12.7±4.5	0.11±0.04
	10	4	12.7±4.5	0.20±0.09
	5	4	12.7±4.5	0.12±0.04