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. Author manuscript; available in PMC: 2008 Sep 12.
Published in final edited form as: J Am Chem Soc. 2005 Dec 14;127(49):17393–17404. doi: 10.1021/ja055672+

Table 2.

Steady-state kinetic parameters for diketide diastereomers and PICS module 2+TEa

Substrate kcat (min−1) Km (mM) kcat/Km (M−1min−1)
syn-(2S,3R)-4 0.61±0.15 4.8±0.19 127±60
anti-(2S,3S)-14 0.027±0.007 7.9±3.2 3.4±1.7
syn-(2R,3S)-13 0.0067±0.0010 2.6±1.2 2.6±1.3
a

Monitored by liquid scintillation counting of combined HPLC-purified triketide acid and triketide lactone products of each incubation.