. Author manuscript; available in PMC: 2008 Sep 12.

Published in final edited form as: J Am Chem Soc. 2005 Dec 14;127(49):17393–17404. doi: 10.1021/ja055672+

Table 3.

PCR primers for site-directed mutagenesis of pTJZ61/PICS module 2+TE.

mutant	primer^a
DH⁰ H3611F	fwd: 5′-CCC GTG GCT CGC GGA CTT CGC CGT CCT GGG GAG CGT CC-3′
DH⁰ H3611F	rev: 5′-GGA CGC TCC CCA GGA CGG CGA AGT CCG CGA GCC ACG GG -3′
KR⁰ S4237A	fwd: 5′- GCG TTC GTC ATG TTC TCC GCC GCC GCC GCC GTC TTC GG -3′
KR⁰ S4237A	rev: 5′- CCG AAG ACG GCG GCG GCG GCG GAG AAC ATG ACG AAC GC -3′
KR⁰ Y4250F	fwd: 5′- GCG GGG CAG GGC GCC TTC GCC GCC GCC AAC -3′
KR⁰ Y4250F	rev: 5′- GTT GGC GGC GGC GAA GGC GCC CTGCCC CGC -3′
KR⁰ K4211Q	fwd: 5′- CAC GTA CTG CGG CCC CAG GTC GAC GCC GCG TTC CTC C -3′
KR⁰ K4211Q	rev: 5′- GGA GGA ACG CGG CGT CGA CCT GGG GCC GCA GTA CGT G -3′

mutant sequences indicated in bold.