Skip to main content
. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Bioconjug Chem. 2007 Dec 12;19(1):158–170. doi: 10.1021/bc7002428

Figure 2.

Figure 2

Digitized image of an IEF gel showing changes in pI when rSAv is modified with the decaborate(2-) derivative 3c to prepare conjugate 4c, and after further modification with succinic anhydride to give 5c. Lanes 1 & 7: IEF standards (Serva Electrophoresis GmbH, Broad pI; 3–10) run in IEF standards run in the direction of application (bottom to top) and opposite direction to application of sample (e.g. top to bottom). Lanes 2 & 6: unmodified rSAv. Lane 3 & 5: r-SAv conjugated with 3c. Lane 4: r-SAv conjugated with 3c and further modified by reaction with an excess of succinic anhydride.