Figure 8.
Accumulation of antigen-experienced T cells and germinal center B cells in Pten and Bim compound heterozygous mice. (a) Flow cytometric analysis of splenic CD4+ T cells and B cells of mice of indicated phenotype. Three mice per genotype were analyzed at the age of 20−28 weeks with similar results. (b) Pten and Bim protein expression in splenic CD4+ T cells from mice of indicated genotypes as determined by immunoblot. (c) Intracellular staining of Pten and Bim protein in splenic CD4+ T cells of the indicated genotypes. MFI, mean fluorescence intensity.