TABLE 2.
The comparison of the S1 and S1′ side chain pocket residues of BoNT E with other serotypes (C/D/G) displaying similarity in P1 and P1′ residue by charge and hydrophobicity, respectively
The S1 pocket also includes zinc and Tyr (Tyr350 in BoNT E), common to all serotypes.
| BoNT E | BoNT C | BoNT D | BoNT G | |
|---|---|---|---|---|
| P1—P1′ | Arg-Ile | Arg/Lys-Ala | Lys-Leu | Ala-Ala |
| S1 pocket | Glu158 | Asp167 | Asp167 | Asp169 |
| S1′ pocket | Thr159 | Pro168 | Tyr168 | Asn170 |
| Phe191 | Leu200 | Leu200 | Asn201 | |
| Thr208 | Ile226 | Ala226 | Thr227 | |
| Met221 | Met221 | Tyr376 |