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. 2008 Sep 19;283(38):26228–26240. doi: 10.1074/jbc.M804858200

FIGURE 3.

FIGURE 3.

Reversible γ-β disulfide bond formation between native γCys-87 and βE381C or βD380C. A, βE381C-F1 was treated with 50 μm CuCl2, and aliquots were taken for SDS-PAGE and ATPase assays (activities listed in Table 1). Lane 1, initial sample, no treatment. Lane 2, βE381C-F1 after 1 h of incubation with CuCl2. Lane 3, βE381C-F1 incubated for 30 min with 10 mm DTT following the reaction with CuCl2. B, βD380C-F1 was treated with 25 μm DTNB and aliquots were taken for SDS-PAGE and ATPase assays (activities listed in Table 1). Lane 1, initial sample, pretreatment. Lane 2, βD380C-F1 after 15 min of incubation with DTNB. Lane 3, βD380C-F1 after 60 min of incubation with DTNB. Lane 4, βD380C-F1 incubated for 30 min with 10 mm DTT after the reaction with DTNB. Note that the gels are slightly overloaded to emphasize the complete disappearance of the γ subunit into the γ-β cross-linked product.