TABLE 2.

Rate constants and kinetically calculated binding constants of cross-linked and noncross-linked enzymes

	k+1	k−1	K1	k+2	k−2	K2	kγ	k+3	k−3	K−3	k+4	k−4	K−4	Turnover (Vmax)	R2	Model selection criteria
	m−1s−1	s−1	m−1s−1	s−1	s−1		s−1	s−1	m−1s−1	m−1	s−1	m−1s−1	m1	s−1
Unisite
Wild typea	1.1 × 105	2.5 × 10−5	4.4 × 109	0.12	0.043	2.8		1.2 × 10−3	4.8 × 10−4	0.4	1.6 × 10−3	1.8 × 102	1.1 × 105
βE381Cb	5.3 × 105	6.0 × 10−4	8.8 × 108	1.2c	1.2c	1.0		2.2 × 10−2	NDd	ND	2.2 × 10−3	ND	ND		0.998c	3.23c
βE381C(γ-β)b	6.1 × 105	3.1 × 10−2	2.0 × 107	1.3c	0.5c	2.6c		2.8 × 10−2	ND	ND	7.7 × 10−3	ND	ND		0.998c	3.96c
βD380C	6.5 × 105	7.3 × 10−2	8.9 × 106	1.8	1.2	1.5		2.5 × 10−3	ND	ND	ND	ND	ND		0.997	3.31
βD380C(γ-β)	1.8 × 105	2.4 × 10−2	7.5 × 106	0.54	0.15	3.6		6.4 × 10−4	ND	ND	ND	ND	ND		0.998	4.27
Multisite
Wild-typee	4.0 × 107	1.9 × 103	2.1 × 104	140	120	1.2	29	210	13	0.062	360	4.2 × 106	1.2 × 104	27	0.997	5.10
βE381C	5.5 × 107	3.7 × 103	1.5 × 104	150	110	1.4	426	220	17	0.077	540	8.2 × 106	1.5 × 104	41	0.998	5.47
βE381C(γ-β)	2.2 × 105	6.1 × 10−3	3.6 × 107	120	80	1.5	0	0 (1.5)f	0	0	0	0	0	(0.44)f	0.958	1.77
βD380C	4.4 × 107	2.1 × 103	2.1 × 104	270	150	1.8	38	267	26	0.1	826	4.0 × 105	4.8 × 102	41	0.999	6.30
βD380C(γ-β)	2.3 × 105	5.2 × 10−3	4.4 × 107	63	17	3.8	0	0 (0.8)f	0	0	0	0	0	(0.16)f	0.972	2.60

^aValues taken from Ref. 14.

^bValues taken from Ref. 28.

^cValues calculated from primary data (28).

^dN.D., not determined.

^eValues taken from Ref. 18.

^fIn parentheses is the value of azide-inhibitable P_i release due to a small fraction of non-cross-linked enzyme. See “Results” for details.