TABLE 6.
Heteromeric assembly of hβ1 mutant subunits with rα1 myc
| Constructa | Heteromeric expressionb | Nc | Homomeric expressiond | N |
|---|---|---|---|---|
| hβ3F | 22 ± 2.2 | (9) | 100 ± 0.9 | (122) |
| hβ1F | 22 ± 3.6 | (7) | 4 ± 0.4 | (109) |
| hβ1F (D37A) | 35 ± 5.5 | (6) | 79 ± 9 | (10) |
| hβ1F* | 13 ± 1.8 | (5) | 4 ± 2 | (5) |
| hβ1F*A | 32 ± 3.9 | (8) | 50 ± 6 | (21) |
| hβ1F*(E172D, G173K) | 28 ± 4.4 | (8) | 5 ± 1 | (5) |
| hβ1F A45V, K197N | 19 ± 2.2 | (5) | 9 ± 1.7 | (3) |
| hβ1F A45V, K196R | 27 ± 7.0 | (6) | 43 ± 3.4 | (4) |
| hβ3F V45A, DNTK | 15 ± 1.8 | (6) | 13 ± 2.8 | (7) |
a
Selected β subunit constructs were co-expressed with rat α1 subunits tagged with the Myc epitope
b
ELISA results for the Myc epitope mean ± S.D., expressed as percent of homomeric hβ3myc assembly
c
Number of separate experiments
d
Data for homomeric expression, extracted from earlier tables