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Sec28p localizes to vesicles on the vacuolar membrane in the ret2-1 mutant. A, ret2-1 cells were shifted to glucose media containing FM for 180 min and examined for Sec28p and FM distributions. B, vacuoles were pre-labeled with FM in ret2-1 cells. Glucose was then added for the indicated times, after which Sec28p and FM distributions were examined.
