FIGURE 9.

FBPase does not co-localize with FM in the Δvam3 mutants, but it co-localizes with endocytic compartments in Δvph1 cells. A, Δvam3 mutants expressing FBPase-GFP were shifted to glucose in the presence of FM for the indicated times. The distribution of FBPase, and FM were visualized and merged. B, Δvph1 cells expressing FBPase-GFP were shifted to glucose in the presence of FM for the indicated times. The distribution of FBPase and FM was examined and merged. C, Δpep4 cells were shifted to glucose for 30 min and lysates were subjected to differentiation centrifugation. High-speed pellets were fractionated on an S-1000 column. Fractions were precipitated with trichloroacetic acid and proteins were resolved using SDS-PAGE. The distribution of FBPase and Pep12p was examined via Western blotting.