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. 2008 Sep 19;283(38):26252–26262. doi: 10.1074/jbc.M802239200

FIGURE 2.

FIGURE 2.

TIRF microscopy of the distribution of YFP-labeled wild type and EF-hand mutant STIM1. A-D, HEK293 cells expressing either YFP-labeled WT STIM1 or YFP-labeled EF-hand mutant STIM1-D76A were examined by TIRF microscopy either immediately before (pre-TG) or at the times shown after the addition of 2 μm thapsigargin (TG). E and F, comparison of HEK293 cells transiently expressing either YFP-labeled WT STIM1 or YFP-labeled STIM1-D76A under identical conditions in store-replete HEK293 cells. G and H, statistical analysis of the relative size of puncta (G) and the overall density of puncta (H) in the images of either STIM1 EF-hand mutant-expressing cells (EF STIM1; from F) or control cells 240 s after thapsigargin (control after TG; from D). Details of the image analysis are given under “Experimental Procedures.”