Single molecule processivity of dynein ATP hydrolysis mutants.
A, kymographs of single molecules of wild-type (WT) or ATP
hydrolysis mutants. The x axis represents the length of an axoneme,
and the y axis shows time. B, velocity histograms of
wild-type and ATP hydrolysis mutants. The mean velocities ± S.D. are
73.9 ± 34.2 nm/s, 4.6 ± 3.7 nm/s, and 60.6 ± 18.9 nm/s
(n = 221, 117, and 384) for wild type, AAA3-E/Q, and AAA4-E/Q,
respectively. C, run length histograms of wild-type and ATP
hydrolysis mutants are distributed in a single exponential decay. Run lengths
were corrected for photobleaching and average axoneme length, and calculations
for correct binning were performed as previously described
(17). Run lengths
(±S.E. as estimated by bootstrapping
(17)) are 2.25 ± 0.14,
1.79 ± 0.18, and 4.38 ± 0.45 μm for wild type, AAA3-E/Q, and
AAA4-E/Q, respectively.