FIGURE 2.

Ers1 is required for processing of centromeric transcripts into siRNAs. A, transcript analysis. RT-qPCR analysis was performed on total RNA extracted from cells of the indicated genotypes constructed in an imr1L::ura4⁺ background. ACT1 was used for normalization. B, siRNA analysis. Small RNAs were analyzed by Northern hybridization using radiolabeled probes against 10 different dg-dh region siRNAs and a control small nucleolar (sno) RNA. A longer exposure is shown to demonstrate the complete lack of siRNAs in the ers1Δ mutant. rel., relative; WT, wild-type; cen, centromeric.