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Effect of GSNL-1 on actin depolymerization. CapZ-capped actin filaments (CapZ: actin = 1:100) were diluted to 0.5 μm actin in the presence of 1 μm latrunculin A, and varying concentrations of GSNL-1 or gelsolin and changes in the intensity of light scattering were monitored for 10 min. GSNL-1 in the presence of 0.1 mm CaCl2 (A), GSNL-1 in the absence of calcium (5 mm EGTA) (B), and gelsolin in the presence and absence of calcium (C) were examined.
