Expression of h2-calponin in myeloid cells. A, Western
blots using anti-h2-calponin polyclonal antibody RAH2 and mAb CP22 on total
protein extracted from human peripheral mononuclear cells, human myelogenous
leukemia cell line K562, human monocyte line THP-1, and in vitro
differentiated human myeloid leukemia cell line HL-60 detected significant
amounts of h2-calponin. B, no calponin expression was detectable in
the human T lymphocyte line Jurkat, mouse T lymphocyte line BW5147, mouse
plasmacytoma cell line T1165, and mouse myeloma cell line NS-1 by RAH2 Western
blot.