TABLE 5.

Liver AdoMet, AdoHcy, and the AdoMet/AdoHcy ratio values in isogenic Shmt1 null mice

Differences between diets were analyzed by Student's t test. Differences among genotypes were analyzed by one-way ANOVA using Tukey's HSD post hoc analysis. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post hoc analysis. Data represent mean ± S.E. values. p values ≤0.05 were considered significantly different. n = 3–7 per group, except n = 2 for Shmt1^–/– mice on the control AIN-93G, as two samples failed during analysis.

Age		22 weeks			32 weeks
Diet	Shmt1 genotype	AdoMet	AdoHcy	AdoMet/AdoHcy	AdoMet	AdoHcy	AdoMet/AdoHcy
		pmol/μg protein	pmol/μg protein		pmol/μg protein	pmol/μg protein
AIN-93G	Shmt1+/+	1.27 ± 0.17	1.39 ± 0.20	1.00 ± 0.16	1.36 ± 0.26	1.21 ± 0.31	1.37 ± 0.33
	Shmt1-/+	1.11 ± 0.15	0.71 ± 0.17	1.81 ± 0.35	1.10 ± 0.22	0.70 ± 0.21	2.16 ± 0.45
	Shmt1-/-	1.44 ± 0.35	0.86 ± 0.10	1.65 ± 0.21	0.85 ± 0.17	0.85 ± 0.30	1.29 ± 0.32
AIN-93G minus folate and choline	Shmt1+/+	0.37 ± 0.04	2.29 ± 0.24	0.17 ± 0.03	0.41 ± 0.05	1.52 ± 0.21	0.27 ± 0.02
	Shmt1-/+	0.69 ± 0.14	1.23 ± 0.20	0.55 ± 0.07	0.60 ± 0.16	0.97 ± 0.14	0.66 ± 0.19
	Shmt1-/-	1.01 ± 0.26	1.37 ± 0.15	0.78 ± 0.18	0.22 ± 0.05	1.00 ± 0.27	0.23 ± 0.04
p value, diet effect		0.0004	0.002	<0.0001	<0.0001	NSa	<0.0001
p value, genotype effect		0.11	0.0005b	0.0009b	NS	NS	0.03c
p value, diet × genotype effect		NS	NS	NS	NS	NS	NS

^aNS means not significant.

^bShmt1^+/+ is significantly different from Shmt1^-/+ and Shmt1^-/-, p < 0.05, as analyzed by one-way ANOVA and Tukey's HSD post hoc test for genotype effect.

^cShmt1^-/+ is significantly different from Shmt1^-/-, p < 0.05, as analyzed by one-way ANOVA and Tukey's HSD post hoc test for genotype effect.