TABLE 7.
Liver uracil content in genomic DNA
Differences between diets were analyzed by Student's t test. Differences among genotypes and genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post hoc analysis. Data represent mean ± S.E. values. p values ≤0.05 were considered significantly different. n = 4–7 per group.
|
Age |
32 weeks, liver uracil |
|
|---|---|---|
| Diet | Shmt1 genotype | |
| pg uracil/μg DNA | ||
| AIN-93G | Shmt1+/+ | 0.13 ± 0.02 |
| Shmt1-/+ | 0.58 ± 0.16 | |
| Shmt1-/- | 0.33 ± 0.08 | |
| AIN-93G minus folate and choline | Shmt1+/+ | 0.36 ± 0.07 |
| Shmt1-/+ | 0.32 ± 0.08 | |
| Shmt1-/- | 0.15 ± 0.02 | |
| p value, diet effect | NSa | |
| p value, genotype effect | 0.08 | |
| p value, diet × genotype effect | 0.03b | |
a
NS means not significant.
b
Shmt1+/+ is significantly different from Shmt1-/+ on control diets, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post hoc test for genotype effect.