TABLE 8.

Metabolic profile of plasma from hypomorphic Shmt1flox/+ mice

Differences between diets were analyzed by Student's t test. Differences among genotypes and genotype × diet effects were analyzed by one or two-way ANOVA using Tukey's HSD post hoc analysis. Data represent mean ± S.E. values. p values ≤0.05 were considered significantly different. n = 5–7 per group.

	Diet						p value
Metabolite	AIN-93G			AIN-93G minus folate and choline
	Shmt1+/+	Shmt1flox/+	Shmt1flox/flox	Shmt1+/+	Shmt1flox/+	Shmt1flox/flox	Diet effect	Genotype effect	Diet × genotype effect
Homocysteine (μm)	4.0 ± 1.6	4.2 ± 1.9	4.2 ± 1.6	5.3 ± 2.2	4.9 ± 2.2	6.3 ± 2.6	0.0003	NSa	NS
Cystathionine (nm)	1120 ± 457	1226 ± 548	1169 ± 442	1391 ± 568	971 ± 434	1228 ± 501	NS	NS	0.05b
Cysteine (μm)	160 ± 65	165 ± 74	182 ± 69	150 ± 61	174 ± 78	177 ± 72	NS	0.007c	NS
α-Aminobutyric acid (μm)	7.4 ± 3.0	9.0 ± 4.0	5.0 ± 1.9	7.8 ± 3.2	6.8 ± 3.1	9.9 ± 4.1	NS	NS	0.03d
Methionine (μm)	59.5 ± 24.3	60.5 ± 27.1	43.1 ± 16.3	44.5 ± 18.2	41.5 ± 18.6	43.4 ± 17.7	0.01	NS	NS
Glycine (μm)	349 ± 143	395 ± 177	348 ± 132	358 ± 146	359 ± 161	314 ± 128	NS	NS	NS
Serine (μm)	185 ± 75	188 ± 84	149 ± 56	161 ± 66	153 ± 69	159 ± 65	NS	NS	NS
Dimethylglycine (μm)	7.78 ± 3.18	6.57 ± 2.94	6.90 ± 2.61	6.37 ± 2.60	6.38 ± 2.85	5.31 ± 2.17	0.05	NS	NS
Methylglycine (μm)	1.39 ± 0.57	1.50 ± 0.67	1.45 ± 0.55	1.48 ± 0.60	1.23 ± 0.55	1.04 ± 0.42	0.03	NS	NS

^aNS means not significant.

^bFolate/choline-deficient Shmt1^+/+ is significantly different from Shmt1^flox/+, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post hoc test for diet × genotype effect.

^cShmt1^+/+ is significantly different from Shmt1^flox/flox, p < 0.05, as analyzed by one-way ANOVA and Tukey's HSD post hoc test for genotype effect.

^dControl diet Shmt1^flox/flox is significantly different from control diet Shmt1^flox/+ and folate/choline-deficient Shmt1^flox/flox, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post hoc test for diet × genotype effect.