Table I.
Summary of epitope characteristics
| Binding Affinity (IC50 nM)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ag Name (peptide position) | VACV-WR Name (peptide position) | Peptide Sequence | Restriction Size | Prediction Ranking | % of Top Scoring Peptides | ELISPOT Assay (SFC/106 cells) | Kd | Ld | Dd |
| NCa | VACVWR148 (598–606) | KYQQDRDTL | Kd, 9-mer | 21 | 0.0 | 365.0 | 6.4 | ND | 53,919 |
| NC | VACWR016 (52–60) | KCENHRSVI | Kd, 9-mer | 282 | 0.5 | 73.3 | 6,995 | ND | — |
| A3L (190–198) | VACVWR122 (190–198) | IYSPSNHHI | Kd, 9-mer | 159 | 0.3 | 632.5 | 1.9 | ND | 17,173 |
| A12L (104–112) | VACVWR131 (104–112) | SYNAMDGQI | Kd, 9-mer | 5 | 0.0 | 177.9 | 1.1 | ND | — |
| A24R (522–530) | VACVWR144 (522–530) | SYFETGFPI | Kd, 9-mer | 43 | 0.1 | 294.6 | 1.1 | ND | 8,768 |
| A24R (489–497) | VACVWR144 (489–497) | SVLSSITNI | Kd, 9-mer | 291 | 0.5 | 85.0 | 76 | ND | 41,894 |
| A33R (103–111) | VACVWR156 (103–111) | LYYQGSCYI | Kd, 9-mer | 38 | 0.1 | 295.0 | 2.8 | ND | — |
| A44L (114–122) | VACVWR170 (114–122) | IYTSSMEAI | Kd, 9-mer | 105 | 0.2 | 106.0 | 2.3 | ND | 35,870 |
| A47L (71–79) | VACVWR173 (71–79) | KIIQKSSSI | Kd, 9-mer | 243 | 0.4 | 56.7 | 191 | ND | — |
| A52R (75–83) | VACVWR178 (75–83) | KYGRLFNEI | Kd, 9-mer | 20 | 0.0 | 1,005.8 | 1.2 | ND | 18,914 |
| B2R (49–57) | VACVWR184 (49–57) | KYMWCYSQV | Kd, 9-mer | 186 | 0.3 | 658.8 | 22 | ND | — |
| B10R (51–59) | VACVWR192 (51–59) | IYKDKQWSI | Kd, 9-mer | 271 | 0.5 | 170.8 | 4,350 | ND | — |
| B16R (191–199) | VACVWR197 (191–199) | YYTCVLEYI | Kd, 9-mer | 283 | 0.5 | 215.2 | 1.3 | ND | 5,767 |
| B19R (304–312) | VACVWR200 (304–312) | SVLTSRGGI | Kd, 9-mer | 270 | 0.5 | 144.0 | 591 | ND | — |
| B22R/B28R/C22L (7–15) | VACVWR004/215 (7–15) | SYILFLSCI | Kd, 9-mer | 22 | 0.0 | 70.0 | 68 | ND | — |
| C6L (74–82) | VACVWR022 (74–82) | GFIRSLQTI | Kd, 9-mer | 258 | 0.4 | 989.4 | 1.4 | ND | 1,153 |
| C10L (159–167) | VACVWR010/209 (159–167) | SYNRSMTTI | Kd, 9-mer | 1 | 0.0 | 115.8 | 1.7 | ND | 34,745 |
| D1R (607–615) | VACVWR106 (607–615) | KYFYGEIAL | Kd, 9-mer | 26 | 0.0 | 70.4 | 36 | ND | — |
| D1R (797–505) | VACVWR106 (797–505) | KFINGASTM | Kd, 9-mer | 28 | 0.0 | 343.3 | 2.2 | ND | — |
| D1R (351–359) | VACVWR106 (351–359) | KYEGPFTTT | Kd, 9-mer | 292 | 0.5 | 47.9 | 2240 | ND | — |
| D4R (29–37) | VACVWR109 (29–37) | FYNEVASWL | Kd, 9-mer | 106 | 0.2 | 330.6 | 1.1 | ND | — |
| D5R (219–227) | VACVWR110 (219–227) | YYFSLQQRL | Kd, 9-mer | 190 | 0.3 | 89.2 | 2.3 | ND | 1,173 |
| D5R (251–259) | VACVWR110 (251–259) | SKIFINSII | Kd, 9-mer | 259 | 0.4 | 518.1 | 87 | ND | 11,247 |
| D5R (140–148) | VACVWR110 (140–148) | TYTTMDTLI | Kd, 9-mer | 91 | 0.2 | 296.8 | 9.4 | ND | 1,368 |
| D13L (61–69) | VACVWR118 (61–69) | QYITALNHL | Kd, 9-mer | 281 | 0.5 | 619.2 | 1.8 | ND | — |
| E2L (149–157) | VACVWR058 (149–157) | SYQFSENEI | Kd, 9-mer | 3 | 0.0 | 45.4 | 1.8 | ND | — |
| E6R (326–334) | VACVWR062 (326–334) | NFINGNTFI | Kd, 9-mer | 167 | 0.3 | 170.4 | 2.1 | ND | 24,184 |
| F1L (86–92) | VACVWR040 (86–92) | LPTKTRSYI | Kd, 9-mer | 268 | 0.5 | 186.0 | 890 | ND | 18,539 |
| F4L (222–230) | VACVWR043 (222–230) | SIITDAVSI | Kd, 9-mer | 290 | 0.5 | 70.0 | 273 | ND | — |
| F11L (158–166) | VACVWR050 (158–166) | LYIPGTSVI | Kd, 9-mer | 2 | 0.0 | 298.3 | 2.3 | ND | — |
| H4L (698–706) | VACVWR102 (698–706) | KYYYSNSYL | Kd, 9-mer | 10 | 0.0 | 157.9 | 1.4 | ND | — |
| I4L (260–268) | VACVWR073 (260–268) | SYISGTNGI | Kd, 9-mer | 16 | 0.0 | 443.8 | 1.4 | ND | — |
| I5L (53–61) | VCAVWR074 (53–61) | KYIGLFIYI | Kd, 9-mer | 11 | 0.0 | 67.1 | 157 | ND | — |
| I8R (511–519) | VACVWR077 (511–519) | SYMKSIQRI | Kd, 9-mer | 13 | 0.0 | 402.1 | 1.1 | ND | — |
| I8R (90–98) | VACVWR077 (90–98) | QYIYSEHTI | Kd, 9-mer | 31 | 0.1 | 617.5 | 2.5 | ND | 21,314 |
| J6R (386–394) | VACVWR098 (386–394) | RYNVIASSI | Kd, 9-mer | 32 | 0.1 | 50.4 | 1.1 | ND | — |
| J6R (874–882) | VACVWR098 (874–882) | KYFFTVSNI | Kd, 9-mer | 7 | 0.0 | 140.0 | 9.4 | ND | 13,876 |
| J6R (782–790) | VACVWR098 (782–790) | KYAANYTKI | Kd, 9-mer | 86 | 0.1 | 492.8 | 1.1 | ND | — |
| N2L (145–153) | VACVWR029 (145–153) | KYKPVYSYV | Kd, 9-mer | 168 | 0.3 | 394.6 | 1.3 | ND | — |
| NC | VACVWR148 (148–156) | IPAALIILL | Ld, 9-mer | 3 | 0.0 | 77.1 | 60 | 55 | 18,926 |
| AORF K (24–32) | A ORF K (24–32) | IPMFNKGHF | Ld, 9-mer | 2 | 0.0 | 85.0 | 45,474 | 78 | — |
| A26L (185–193) | VACVWR149 (185–193) | SPMYLWFNV | Ld, 9-mer | 32 | 0.1 | 45.2 | 17,159 | 11 | 13,672 |
| C9L (379–387) | VACVWR019 (379–387) | MPLFPTFSM | Ld, 9-mer | 8 | 0.0 | 84.0 | 35,852 | 18 | — |
| E9L (716–724) | VACVWR065 (716–724) | NPLSNPFYM | Ld, 9-mer | 16 | 0.0 | 222.3 | 11,740 | 1.6 | 13,144 |
| F2L (26–32) | VACVWR041 (26–32) | SPYAAGYDL | Ld, 9-mer | 14 | 0.0 | 900.6 | 27,686 | 0.38 | 32,218 |
| I6L (159–167) | VACVWR075 (159–167) | IPMSIISFF | Ld, 9-mer | 5 | 0.0 | 90.4 | 9,587 | 2.3 | 8,059 |
| I8R (340–348) | VACVWR077 (340–348) | LPNPAFIHI | Ld, 9-mer | 15 | 0.0 | 140.6 | 16,890 | 1.0 | 27,134 |
| J4R (157–165) | VACVWR096 (157–165) | IPLKIVRFF | Ld, 9-mer | 13 | 0.0 | 92.5 | 40,825 | 20 | 39,317 |
| L3L (221–229) | VACVWR090 (221–229) | LPQSCYLNF | Ld, 9-mer | 4 | 0.0 | 175.0 | 42,762 | 7.4 | 6,537 |
| M1L (376–384) | VACVWR030 (376–384) | IPFIAYFVL | Ld, 9-mer | 7 | 0.0 | 135.0 | 63,859 | 1.5 | 48,588 |
| O1L (210–218) | VACVWR068 (210–218) | YPMDNVINF | Ld, 9-mer | 6 | 0.0 | 100.4 | 4,255 | 10 | 59,978 |
| A35R (31–39) | VACVWR158 (31–39) | IGPYIIGNI | Dd, 9-mer | ND | ND | 167.7 | 61,401 | ND | 0.55 |
| E3L (140–148) | VACVWR059 (140–148) | VGPSNSPTF | Dd, 9-mer | ND | ND | 771.0 | — | ND | 0.93 |
| G9R (68–77) | VACVWR087 (68–77) | TGPGGLSALL | Dd, 10-mer | ND | ND | 37.3 | — | ND | 8.6 |
Dashes indicate IC50 > 70000 nM
a
NC, No classification; ND, no data available.