Figure 1.

Plasma membrane coated pit distribution in cells overexpressing a chimeric receptor containing a tyrosine-based internalization signal. Confocal immunofluorescence microscopy images showing distribution of the Tac reporter (left panels) and AP2 (right panels) visualized using rabbit anti-Tac serum and monoclonal AP.6, respectively, in Tac and TTM.GSTYΔ transiently transfected HeLa cells. Images were taken with a focal plane parallel to and near the substrate. Note AP2 staining in both transfected and untransfected cells. Bar, 20 μm.