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Coated pit distributions in cells overexpressing chimeric receptors containing di-leucine- or acidic cluster-based internalization signals. Confocal microscopy ‘x-y’ images showing the distribution of CPs (AP2, right panels) in HeLa cells transiently transfected to overexpress Tac, TTγt3-t2, and TacF766–93 (Tac, left panels). Images were acquired using a focal plane parallel to and near the substrate. Note AP2 staining in both transfected and untransfected cells. Bar, 20 μm.
