Overexpression of chimeric receptors containing tyrosine-, di-leucine-, or acidic cluster/casein kinase II-based endocytic motifs does not affect the perinuclear localization of the Golgi- associated AP1. Distribution of AP1 visualized with 100/3 mAb (right panels) in HeLa cells in which expression of TTM.GSTYΔ (upper panels), TTγt3-t2 (middle panels), or TacF766–93 (lower panels) is revealed by staining with anti-Tac antibody (left panels). Note the AP1 distribution in both transfected and untransfected cells. Bar, 20 μm.