Figure 3.

Katanin-mediated flagellar microtubule severing is supported by ATP but not by either ADP or ATPγS. The percentage of flagella scored in each of the categories illustrated in Figure 3 was determined in the presence of katanin (100 μg/ml) plus the indicated nucleotide (2.5 mM). As shown, katanin-induced flagellar axonemal microtubule kinking and breakage was supported by ATP (A) but not by ADP or ATPγS (B and C). Bars represent the average ± SE, n = 4; 50 cells counted for each experiment. All other conditions as in Figure 2.