Figure 5.

Katanin induces kinking and severing of fa1–1 flagella. NP-40 (0.05%)-permeabilized fa1 mutant cells (≈1 × 10⁵ cells/ml) were incubated with purified katanin (100 μg/ml) plus 5 mM ADP (A) or 5 mM ATP (B–D) for 10 min. In the presence of ATP, katanin causes kinking (B), severing (C), and deflagellation (D) of fa1–1 flagella. (E) Flagellar preparations made from the katanin-treated fa1 cells consisted of small, kinked flagellar fragments resembling isolated wild-type flagella treated with katanin (Figure 2F).