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Anti-human p60 antibodies strongly recognize a ≈55-kDa Chlamydomonas protein in both whole-cell and flagellar–basal body protein preparations. Whole-cell and FBBC proteins (10 μg) were separated on a 4–15% SDS-PAGE gel and analyzed by Western blot with an affinity-purified antibody to the 60-kDa subunit of human katanin. Detection was with an alkaline phosphatase-conjugated secondary antibody.
