Figure 4.

PI 3-kinase activity in vehicle- and DA-treated PCT cells was determined in immunoprecipitates with an anti-p85α and anti-phosphotyrosine antibody. Only the p85α revealed PI 3-K activity. Controls were performed in PCT cells treated with insulin-like growth factor I (100 ng/ml, 2.5 min) and insulin (10 nM, 2.5 min). Ins, insulin.