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. 1998 May;9(5):1221–1233. doi: 10.1091/mbc.9.5.1221

Figure 1.

Figure 1

Bni1p–Spa2p interactions in the yeast two-hybrid method. Plasmids encoding ADGAL4 and DBDLexA fused to truncated Bni1ps were transformed into strain L40 expressing DBDLexA-Spa2p(1213–1466) and ADGAL4-Spa2p(1213–1466), respectively. Bni1ps-Spa2p(1213–1466) interactions were examined in each transformant by the qualitative and quantitative assay methods for β-galactosidase activity. Closed bars, blue colony color; open bars, white colony color. The values (Miller units) are the averages of β-galactosidase activities for three transformants. Each measured value was within 50% of the average. FH1 and FH2 are formin homology domains 1 and 2, respectively. (A) Interactions of Bni1ps with DBDLexA-Spa2p(1213–1466). (B) Interactions of Bni1ps with ADGAL4-Spa2p(1213–1466).