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SPARC-null and wild-type adult skin fibroblasts change shape in response to rSPARC. Wild-type (A, B, E and F) and SPARC-null fibroblasts (C, D, G and H) were cultured on glass coverslips in the absence (A–D) and presence of 0.9 μM rSPARC (E–H). (A, C, E, and G) BODIPY-conjugated phalloidin was used to visualize the actin cytoskeleton. (B, D, F, and H) Anti-SPARC antibody was used to detect SPARC immunoreactivity. Bar, 5 μm.
