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IRAP has identical sedimentation distribution as GLUT1 and GLUT4 and is insulin responsive by day 3 of differentiation. Various conditions are as described in Figure 4, except that even- and odd-numbered fractions were immunoblotted for IRAP on days 3 (A and B) and 9 (C and D), respectively. Also shown (E) is a representative profile (O.D. 562) of the total protein (triangles) in these gradients (Figures 4–6), which does not change during differentiation or upon insulin treatment.
