Figure 1.

The unc-64 syntaxin locus. (A) Similarity among syntaxin molecules. An alignment of syntaxin family proteins from C. elegans, Drosophila (Schulze et al., 1995), and human (Osborne et al., 1997). The position of the molecular lesions identified in selected mutations in the unc-64 syntaxin gene (bold) and the resulting change in amino acid sequence are indicated above an arrow. The standard single-letter amino acid code is used. Dots represent identity with the C. elegans sequence. Amino acid number appears at the right. (B) Restriction map of the genomic unc-64 gene. Below the restriction map is a schematic diagram showing the intron–exon structure of three unc-64 products and the sequences contained in plasmid constructs used in this study. Gray represents predicted message structures. C, NcoI; X, XhoI; N, NheI; P, PstI restriction endonuclease sites. (C) Autoradiograph of a Northern blot probed with a syntaxin cDNA fragment; 10 μg of mixed-staged poly A(⁺) selected RNA isolated from wild-type hermaphrodites was loaded on the gel. RNA size standards (kb) are on the right.