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WRS-85D has aminoacylation activity. Bacterially purified WRS-85D was incubated with total tRNA and [14C]l-tryptophan (A) or [14C]l-leucine (B). Protein purified from cells containing the expression vector pTrcHisB alone was used as a negative control. Reticulocyte lysate was used as a positive control. ♦, WRS-85D; □, reticulocyte lysate; ▵, pTrcHisB. Data are the mean ± SD of three independent experiments for each sample.
