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. 1998 Jun;9(6):1253–1263. doi: 10.1091/mbc.9.6.1253

Figure 2.

Figure 2

Ammonium-induced degradation of Gap1 is dependent on vacuolar proteases. (A) Cells were grown on proline medium, and Gap1 activity was measured by incorporation of [14C]citrulline (0.1 mM) before (t = 0) and at several times after addition of (NH4+)2SO4 (10 mM) in strains RTY1 (pep4Δ; •) and 27061b (wild type; □). The Gap1 activities were calculated in nanomoles per minute per milliliter. (B) Immunoblot of Gap1 in crude extracts prepared before (t = 0) and at several times after addition of (NH4)2SO4.