Figure 5.

E1-G_CT colocalizes with proteins involved in generation of ER-derived transport vesicles. BHK-E1-G_CT cells were fixed with methanol as described above and incubated with rabbit (A) and mouse (C) antibodies directed against the ERGIC marker p58 or rabbit anti-Sec23p (E). E1-G_CT was detected using P5D4 (B), human anti-E1 (D), or rabbit anti-G_CT (F). The distribution of Sec23p (G) and mouse anti-G_CT (H) in untransfected BHK cells are also shown. Secondary antibodies were FITC-donkey anti-rabbit IgG, FITC-goat anti-human IgG, and Texas Red-goat anti-mouse IgG. Overlap between p58 and Sec23p with E1-G_CT in the juxtanuclear region is indicated by arrowheads. Bar, 10 μm.