Figure 6.

Metabolic labeling and immunoprecipitation of αF-G′ from internal and external cellular fractions. G2-11 and GPY1452 cells expressing αF-G′ were labeled as described in Figure 2A. Cells were converted to spheroplasts, and the released periplasmic fraction was combined with the culture supernatant to produce the external (E) fraction. Spheroplasts were lysed to generate the internal (I) fraction. Proteins were immunoprecipitated and treated with Endo H as described in Figure 2B before being subject to SDS-PAGE.