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. 1998 Jun;9(6):1367–1378. doi: 10.1091/mbc.9.6.1367

Figure 4.

Figure 4

Rho-V14 overexpression, as well as Rac-V12 overexpression, prevents forskolin-induced dendricity. (A) B16 melanoma cells were transiently transfected with an expression plasmid encoding the constitutively active mutant of RhoA (pEXVRhoA-V14) tagged with the myc epitope. After transfection, cells were incubated in serum containing medium (NS; a and b) or in serum containing medium with 20 μM forskolin (FK; c and d). Cells were then labeled with the monoclonal anti c-myc antibody 9E10 to detect Rho-V14-expressing cells (a and c) or with phalloidin-rhodamine to detect the F-actin (b and d). Arrows in c and d, Rho-V14–expressing cells. Bar, 60 μm. (B) Cells were transfected with a vector encoding the constitutive active form of Rac (pEXVRac-V12) tagged with the myc epitope. Cells were then incubated in serum containing medium (NS; a and b) or in serum containing medium with 20 μM forskolin (FK; c and d). Cells were then labeled with the monoclonal anti c-myc antibody 9E10 to detect Rac-V12-expressing cells (a and c) or with phalloidin-rhodamine to detect the F-actin (b and d). Arrows in b and d indicate cortical actin in lamellipodia structures in Rac-V12–expressing cells. Bar, 40 μm.