Figure 5.

Constitutively active P160^ROCK overexpression prevents dendrite outgrowth. Growing B16 cells were transiently transfected with the pCAGGS vector containing the cDNA encoding a constitutively active form of P160^ROCK tagged with the myc epitope. After transfection, cells were incubated in fresh medium (NS; a and b) or fresh medium containing 20 μM forskolin (FK; c and d) for 12 h. Then cells were stained with the anti-myc antibody 9E10 to detect P160^ROCKexpressing cells (a and c) or with phalloidin-rhodamine to detect F-actin (b and d). Arrows indicate active P160^ROCK expressing cells. Bar, 30 μm.