Table 1.
ORFa | Gene | Function | Fold induction | ZRE | |||||
E1b | E2b | E3-1c | E3-2c | startd | scoree | sequence | |||
Experimentally tested Zap1 target genes | |||||||||
YDR284C | DPP1 | diacylglycerol pyrophosphate phosphatase | 5.5 | 3.6 | 2.4 | 2.2 | -452 | 9.6 | ACCTGAAAGGT |
YDR492W | IZH1 | membrane steroid hormone receptor ortholog | 2.8 | 4.4 | 1.4 | 1.6 | -914 | 5.0 | TCCCTCAATGA |
-417 | 11.2 | ACCCTAAAGGT | |||||||
YGL255W | ZRT1 | plasma membrane zinc uptake transporter | 24.2 | 18.9 | 38.8 | 34.4 | -456 | 9.3 | ACCTTTGGGGT |
-338 | 7.7 | ACCTCGAAGGA | |||||||
-319 | 12.0 | ACCTTGAGGGT | |||||||
-204 | 11.7 | ACCTTGAAGGT | |||||||
YGL256W | ADH4 | alcohol dehydrogenase IV | 26.2 | 5.6 | 12.6 | 12.6 | -269 | 9.4 | ACCGTGAAGGT |
-191 | 5.3 | GCCTTCAATGA | |||||||
YJL056C | ZAP1 | zinc-responsive transcriptional activator protein | 7.5 | 16.9 | 96.5 | 79.7 | -144 | 11.2 | ACCCTAAAGGT |
YKL175W | ZRT3 | vacuolar zinc export transporter | 7.9 | 8.7 | 4.9 | 4.8 | -155 | 11.6 | ACCTTAAGGGT |
YLR130C | ZRT2 | plasma membrane zinc uptake transporter | 5.6 | 12.3 | 2.7 | 2.5 | -941 | 6.1 | ACCCTAACTGT |
-311 | 11.2 | ACCCTAAAGGT | |||||||
-262 | 11.2 | ACCCTAAAGGT | |||||||
-174 | 5.8 | ACCTTTTGGGA | |||||||
-112 | 4.9 | ACCAACAGGGT | |||||||
-41 | 5.3 | GCCTGCAATGT | |||||||
YMR243C | ZRC1 | vacuolar zinc import transporter | 2.1 | 4.7 | 1.6 | 1.8 | -174 | 9.6 | GCCTTGAAGGT |
YMR319C | FET4 | plasma membrane Fe/Cu/Zn uptake transporter | 2.3 | 2.4 | 1.6 | 1.7 | -384 | 7.8 | ACCCCACGGGT |
YNR039C | ZRG17 | endoplasmic reticulum zinc import transporter | 3.2 | 3.5 | 2.3 | 1.9 | -527 | 11.7 | ACCTTGAAGGT |
YOL002C | IZH2 | membrane steroid hormone receptor ortholog | 1.9 | 2.0 | 3.4 | 3.3 | -256 | 7.7 | ACCCTAGAGGA |
-173 | 7.2 | ACCCCGAGTGT | |||||||
YOL154W | ZPS1 | cell wall protein, putative metalloprotease | 13.9 | 10.1 | 78.6 | 57.9 | -329 | 11.8 | ACCTTCAGGGT |
-314 | 11.8 | ACCTTCAGGGT | |||||||
Likely Zap1 target genes | |||||||||
YBL049W | MOH1 | zinc-binding protein, function unknown | 10.7 | 2.2 | 3.2 | 3.8 | -387 | 8.7 | CCCTTGAGGGA |
YBR117C* | TKL2* | transketolase, pentose phosphate pathway | 5.4 | 2.0 | 1.9 | 2.1 | -844 | 8.2 | ACCTTATGGGT |
-524 | 5.3 | ACCCAAAATGT | |||||||
YDR055W* | PST1* | cell wall protein | 5.0 | 2.7 | 2.0 | 1.9 | -886 | 7.4 | ACCCCAAGGGA |
-405 | 8.0 | TCCTTGAGGGA | |||||||
YGL121C | GPG1 | putative gamma subunit of a heterotrimeric G protein | 10.8 | 2.7 | 2.1 | 2.4 | -287 | 8.3 | CCCTTCGAGGT |
-208 | 9.0 | ACCGTAAAGGT | |||||||
YGL257C | MNT2 | mannosyltransferase, O-linked glycosylation | 2.3 | 4.4 | 4.0 | 3.7 | -572 | 5.3 | GCCTTCAATGA |
-494 | 9.4 | ACCGTGAAGGT | |||||||
YGL258W | VEL1 | function unknown | 16.3 | 4.0 | 112.6 | 78.6 | -763 | 6.3 | ACCTTGCATGA |
-232 | 10.4 | ACCCTGCGGGT | |||||||
YGR295C | COS6 | function unknown | 2.7 | 2.2 | 1.5 | 1.6 | -875 | 5.0 | AACTTAAATGT |
-309 | 9.1 | ACCTTAAATGT | |||||||
YJL132W* | similar to phospholipase D | 5.2 | 3.8 | 3.3 | 2.8 | -154 | 7.8 | ACCCAAAGGGT | |
YJR061W | Mnn4-related, N-linked glycosylation | 4.6 | 2.1 | 2.0 | 1.8 | -278 | 9.8 | ACCTTCAAGGA | |
YKL165C | MCD4 | glycosylphosphatidylinositol (GPI) anchor synthesis | 4.1 | 2.5 | 1.8 | 1.8 | -104 | 11.4 | ACCTTAAAGGT |
YLL020C | function unknown | 5.1 | 3.1 | 1.7 | 1.6 | -933 | 12.0 | ACCTTGAGGGT | |
YMR120C | ADE17 | AICAR transformylase, purine biosynthesis | 5.2 | 3.7 | 1.9 | 2.2 | -98 | 8.7 | ACCTTTAGTGT |
YMR271C | URA10 | orotate phosphoribosyltransferase, pyrimidine biosynthesis | 6.1 | 2.5 | 4.9 | 5.2 | -143 | 7.9 | ACCTTTCGGGA |
YMR297W | PRC1 | vacuolar carboxypeptidase Y | 3.0 | 3.5 | 1.5 | 1.9 | -182 | 8.1 | ACCCCGCGGGT |
YNL254C | function unknown | 9.7 | 12.0 | 2.3 | 2.0 | -432 | 11.7 | ACCTTGAAGGT | |
YNL336W | COS1 | function unknown | 2.1 | 2.0 | 1.6 | 1.6 | -871 | 5.0 | AACTTAAATGT |
-591 | 5.3 | AACCTAGAGGT | |||||||
-314 | 9.1 | ACCTTAAATGT | |||||||
YOL084W | PHM7 | major facilitator superfamily member, function unknown | 9.7 | 2.1 | 2.5 | 2.3 | -766 | 11.7 | ACCTTGAAGGT |
YOL131W | function unknown | 5.0 | 2.3 | 2.5 | 2.2 | -1000 | 7.6 | AACTTCAGGGT | |
-458 | 8.2 | ACCTGGAAGGA | |||||||
-364 | 5.9 | ACCCAAGAGGT | |||||||
-122 | 5.5 | TCCATTAGGGT | |||||||
YOR387C | function unknown | 17.8 | 19.3 | 136.4 | 113.6 | -763 | 6.3 | ACCTTGCATGA | |
-231 | 10.4 | ACCCTGCGGGT | |||||||
YOR134W | BAG7 | GTPase-activating protein, control of cell wall synthesis | 5.9 | 2.0 | 1.7 | 1.6 | -445 | 6.0 | CCCTCCAAGGA |
-325 | 8.6 | CCCCTGCAGGT | |||||||
YPL250C* | ICY2* | function unknown | 2.1 | 2.3 | 3.1 | 3.1 | -756 | 8.9 | CCCTTCCGGGT |
-524 | 7.4 | ACCCCAAGGGA | |||||||
-475 | 5.7 | GCCCAAAGGGT | |||||||
-360 | 5.6 | CCCGTCAGTGT |
a) Results for genes marked with asterisks were confirmed independently by S1 nuclease protection assay in Figure 2.
b) Expression ratios are the average of two independent microarray experiments (Lyons et. al. 2000).
c) Results from two independent microarray experiments (E3-1 and E3-2) are shown.
d) Numbers indicate the distance from the ATG initiation codon.
e) Score calculated for each sequence with a position-specific scoring matrix generated by RSAT.