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. Author manuscript; available in PMC: 2008 Sep 12.
Published in final edited form as: Anal Chem. 2007 May 25;79(12):4666–4673. doi: 10.1021/ac0618730

Figure 4. Comparison of different resins in the ability to enrich phosphopeptides from a tryptic digest of α and β-casein.

Figure 4

Results shown are the average of duplicate runs, loading 1 pmol (of each digested protein). The optimized buffer system using online two-dimensional liquid chromatography, as described in the text, was used for all chromatography runs except for the RP-only run (reversed phase only, single dimension, where no enrichment step is performed). All reported values represent average spectra counts or ratios of average spectra counts from duplicate runs and are normalized to the RP-only run, additional data presented in S-Table 11.