. Author manuscript; available in PMC: 2008 Sep 12.

Published in final edited form as: Anal Chem. 2007 May 25;79(12):4666–4673. doi: 10.1021/ac0618730

Table 1.

Results of the use of different concentrations of acetonitrile (ACN) in loading and wash buffers with 0.1% formic acid (FA) in a TiO₂-based enrichment of phosphopeptides from a tryptic digest of α and β-casein.

	% ACN	Peak area

		K.YKVPQLEIVPNS*AEER.L	K.FQS*EEQQQTEDELQDK.I

0.1% FA	0	1.7E+09	7.5E+09
	5	2.8E+09	1.3E+10
	10	5.6E+09	1.8E+10
	20	1.1E+10	1.9E+10
	40	1.0E+10	1.8E+10
	80	4.4E+09	2.1E+10