Figure 4.—

P_lag-2-induced FLP-out induces lacZ expression in the somatic gonad lineage. (A) β-gal expression in the DTC only; FLP-out occurred after the DTC was born. (B) β-gal expression in the entire somatic gonad; FLP-out occurred in the early L1 stage (prior to the division of Z1 or Z4). Worms were of the genotype naEx57[P_lag-2∷FLP, P_ceh-22∷GFP]; naEx64[P_pro-1<GFP<lacZ]. Both images were captured under DIC optics, and worms were stained with X-gal staining solution to visualize β-gal. Arrowheads indicate the DTC (A) and gonadal sheath cells (B) where FLP-out occurred. Bar, 50 μm.