Figure 6.—

Conditional expression of HLH-12(R25K) and induction of hlh-12(RNAi) by FLP-out. (A) DIC image of an L2 worm of the genotype naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx164[P_hlh-12<GFP<hlh-12(R25K)] . (B) GFP image of the same worm as in A. GFP expression driven by P_hlh-12 in the DTC (asterisk). (C) Non-heat-shocked naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx164 [P_hlh-12<GFP<hlh-12(R25K)] worm that did not display the Mig phenotype. (D) Heat-shocked naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx164 [P_hlh-12<GFP<hlh-12(R25K)] worm that exhibited the Mig phenotype. (E) DIC image of an L4 worm of the genotype naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx168[P_hlh-12<GFP<hairpin_hlh-12)]. (F) GFP image of the same worm as in E; GFP expression driven by P_hlh-12 in the DTC (asterisk). (G) Non-heat-shocked naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx168[P_hlh-12<GFP<hairpin_hlh-12)] worm that did not display the Mig phenotype. (H) Heat-shocked naIs6[P_hsp-16.2∷FLP, P_ceh-22∷GFP]; naEx168[P_hlh-12<GFP<hairpin_hlh-12)] worm that exhibited the Mig phenotype. Bar, 50 μm.