Figure 8.—

ChIP analysis of histones and histone modifications at EPgy2 insertion sites. Antibody quantification was performed using real-time PCR with primers specific to the promoter of the mini-w transgene and the 3′ end of the EPgy2 insertion. Graphs show real-time PCR results. The level of each antibody was normalized to 5% of input DNA and further normalized to the RpL32 locus. The data were obtained from four to six independent experiments, each of which included all of the strains compared. EY00630 carries an insertion in euchromatin of chromosome 2R. The EY00453 insertion is located in 3L HTT, EY03383 carries an insertion in 2R TAS, and the EY06734 insertion is located in 2R pericentric heterochromatin. Error bars represent standard deviations.