Figure 2.—

Fruiting body development and ascospore germination of wild type, Δgsa, and Δsac1 mutant strains. r2 and fus1 are spore color mutants that show wild-type fertility. (A) Perithecia on solid cornmeal medium after 11 days of growth. The scale bar represents 1 mm. (B) Ascospore germination of wild-type and mutant strains Δsac1 and Δgsa3/r2. Spores were incubated for 5 hr on solid cornmeal medium with 0.5% (w/v) sodium acetate. The scale bar represents 50 μm. (C) Lateral view on perithecia of wild type, Δsac1, Δsac1/fus1 supplemented with cyclic AMP (+cAMP), and retransformant Δsac1 + cr-1 on solid cornmeal medium after 9 days of growth. The scale bar represents 200 μm.