Figure 3.—

(A–C) A 30-hr pupal wing stained with both anti-Mwh (red) and anti-Stan (green) antibodies. As in Figure 2 single-channel images are shown in grayscale to improve contrast. B is the merged image. Note the Stan staining is more precisely localized at the plasma membrane and the two proteins are not tightly colocalized. (D–F) A 30-hr pupal wing stained with both anti-Mwh (red) and anti-In (green) antibodies. In immunostaining is very sensitive to fixation so only very weak fixation was used in this experiment and this led to the poor anti-Mwh staining. (I and K) A wing bristle where the endogenous Mwh protein is immunostained (red). F-actin is in green. (J and L) Higher magnification images of the proximal region of the bristle. Note that Mwh accumulates along the large actin bundles and is preferentially in the proximal part of the bristle. M–O) A piece of larval body wall muscle where mwh expression was driven using Tubulin-Gal4. Mwh is shown in red and F-actin in green (Alexa 488 phalloidin). Note the strong Mwh staining in the gap between regions of F-actin staining.