Figure 3.—

ibr1 and ibr10 mutants metabolize peroxisomal substrates similarly to wild type. (A) ibr mutant hypocotyl elongation in the absence of sucrose. Seeds were stratified for 4 days at 4° in 0.1% agar prior to plating on unsupplemented medium or medium containing 0.5% sucrose. Plates were placed under white light for 1 day at 22° and then transferred to the dark at 22° for 5 additional days. Error bars show standard errors of mean hypocotyl lengths (n ≥ 10). chy1-3 (Zolman et al. 2001a) is included as a sucrose-dependent control. (B) ibr mutant root elongation on propionate and isobutryate. Seeds were stratified for 3 days at 4° in 0.1% agar prior to incubation under white light for 2 days at 22°. Germinating seeds were transferred to medium containing 0.5% sucrose with or without 150 μm propionate or 350 μm isobutyrate. Roots were measured after 7 additional days of growth. Error bars show standard errors of mean root lengths (n ≥ 11). chy1-3 is included as a propionate- and isobutyrate-hypersensitive control (Lucas et al. 2007).