Figure 5.—

Most l(3)psg mutants display defects in rpr and hid expression. RNA was extracted from salivary glands of staged control animals at either puparium formation (−12 hr) or 1.5 hr AHE (1.5 hr) and from salivary glands of each l(3)psg mutant staged at 6 hr AHE. RNA was analyzed by Northern blot hybridization for levels of rpr and hid transcript. Both rpr and hid are induced by 1.5 hr AHE in control salivary glands, in response to the prepupal pulse of ecdysone that triggers pupation. Mutants l(3)psg1, l(3)psg2, l(3)psg3, l(3)psg4, and l(3)psg5 show defects in induction in one or both death activators, while l(3)psg6 and l(3)psg7 do not show significant changes in death activator expression. Hybridization to detect rp49 mRNA was used as a control for loading and transfer.